DNA

Part:BBa_K4177006

Designed by: Junchen Dong   Group: iGEM22_HiZJU-China   (2022-10-08)


dCas9

dCas9 protein is a mutant of Cas9 protein, in which two nuclease active regions, RuvC1 and HNH, are mutated at the same time. Thus, the endonuclease activity of the dCas9 protein is completely eliminated, leaving only the ability to be guided into the genome by the gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None